This study was conducted to develop an efficient protocol for mass propagation of Hedychium gardnerianum. Explants from rhizome buds were cultured on Murashige and Skoog (MS) medium supplemented with 6-Benzylaminopurine (BAP) and indole 3-acetic acid (IAA) alone (0.1 to 5 mg l-1) or a combination of BAP and IAA. MS medium supplemented with a combination of 0.5 mg l-1 BAP and 0.1 mg l-1 IAA produced the highest mean number of shoots (5) and highest number of roots (3.88) per explant as compared to other concentrations. Thus, combined effects of BAP and IAA improved significantly the shoot growth and proliferation. The proliferated shoots were green and healthy in appearance. Finally, healthy and complete plants with well developed roots were hardened, acclimatized and planted in the field successfully.
Keywords Hedychium gardnerianum, micropropagation, in vitro, acclimatization, ornamental plants.